5-mC DNA ELISA Kit (1 x 96) and ,货号-规格：D5325-1组

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5-mC DNA ELISA Kit (1 x 96) and ,货号-规格：D5325-1组

HIGHLIGHTS

For high-throughput detection of global 5-methylcytosine (5-mC) in DNA
The streamlined workflow can be completed in less than 3 hours

DESCRIPTION

The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as methylated PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.

TECHNICAL SPECIFICATIONS

Detection	> 0.5% 5-mC per 100 ng DNA
Equipment	Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.
Input	This protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng/well.
Sample Source	Purified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar.

KIT COMPONNTS

Cat #	Name	Size
D5325-1-15	5-mC Coating Buffer	15 ml
D5325-1-30	5-mC Coating Buffer	30 ml
D5325-3-15	Secondary Antibody	15 µl
D5325-3-30	Secondary Antibody	30 µl
D5325-2-250	5-mC ELISA Buffer	250 ml
D5325-5-1	Negative Control	50 µl (100 ng/µl)
D5325-5-2	Positive Control	50 µl (100 ng/µl)
D5425-4-15	HRP Developer	15 ml
D5425-4-30	HRP Developer	30 ml
A3001-30
A3002-15	Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21)	15 µl
C2020	96-Well ELISA Plate	12 x 8-Well Strips