- 产品详情
- 产品参数
- 说明书下载
- 手机版
- 参考文献
Foxp3 / Transcription Factor Fixation/Permeabilization Buffer Set,货号-规格：FXP058-100 tests
DESCRIPTION:
FOXP3 Fix/Perm Buffer Set has been specially formulated for intracellular staining FOXP3 with minimum effect on the surface fluorochrome staining. It contains one 30 ml 4X concentrated FOXP3 Fix/Perm buffer and one 25 ml 10Xconcentrated FOXP3 Perm buffer . The FOXP3 Fix/Perm buffer(4X) must be freshly diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer (4X) to three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted to 1X workingsolution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Perm buffer(10X) to nine (9) parts PBS.
STORAGE:2°C and 8°C. Do not freeze.
Recommended Usage ：The FOXP3 Fix/Perm buffer(4X) must be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Fix/Perm buffer(4X) to three (3) parts PBS. The FOXP3 Perm buffer (10X) should be diluted to 1X working solution with PBS (10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2) prior to staining procedures. e.g. Dilute one (1) part FOXP3 Perm buffer(10X) to nine (9) parts PBS.NOTE: The FOXP3 Perm buffer (10X) may have crystalization or precipitation oberserved when it is stored at 2-8°C, however, it's normal and does not affect the buffer performance. If there is a heavy precipitation observed after diluted to 1X working solution, it can be filtered to clarify the solution.