实验原理

先通过电泳使不同蛋白按分子量大小在聚丙烯胺凝胶中依次排列，然后将凝胶中的蛋白质转移至转印膜上，随后用封闭剂覆盖转印膜上未吸附目标蛋白质的区域，最后通过"抗原-抗体"免疫学反应来检测目标蛋白的表达水平。

蛋白质免疫印迹（WB）实验步骤

1、需要告知样本种属、样本形式、数量、编号、内参、上样顺序、检测指标、整膜等信息，详细填写《雅酶实验代做委托表》（此表请打印后随样品一起寄出）;

2、样本∶每孔总的上样量是20微克；组织约黄豆大小，质量至少100-200mg/样，干冰运输;常规细胞量不少于2×106个，若检测指标过多，需要适当增加样本量

3、提供的样品不能石蜡包埋过或泡过多聚甲醛 /TRIZOL;

4、客户可以自行提供目标蛋白和一抗，也可以在本公司订购或由我司代买;10uL/张/指标；抗体尽量原包装不要分装，多余可以返还。

5、需确保提供的实验材料安全无毒、无致病性、无传染性，对人、动植物和环境友好;

6、价格、特殊样本及其他要求需单独咨询。交付周期一般收到样本后2周交付（着急可提前沟通交付时间）

7、WB、ELISA、样本需要干冰运输，特殊染色（冰冻切片需干冰运输）其余常温运输；

更多技术服务敬请期待哦...

Western-Blot检测细胞中 NF-kB P65，NF-kB pP65(S536)，nlrp3的含量

1	2	3	4	5	6
						NF-kB P65
						NF-kB pP65
						nlrp3
						β-actin

精品重组抗体，实事求是做好每一次验证实验！14000+抗体，20µL/50µL/100µL可选，可指导实验，可寄样优化条件，认真对待您的每一次体验！

点击二维码查看雅酶抗体列表‍

Anti-Phospho-Histone H2A.X (Ser139) Rabbit mAb [15L14K33]

Western Blot-Anti-Phospho Histone H2A.X(Ser139]Rabbit mAb [15L14K33]

All lanes: R010101 at 1:5,000 dilution

Lane 1: Rat spleen whole tissue lysates

Lane 2: Rat heart whole tissue lysates

Lysates/proteins at 10 ug per lane.

Secondary antibody: Goat Anti-Rabbit lgG(H+L)

HRP Conjugated

(Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 15kDa

Observed band size: 15~17kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry-Anti-Phospho-Histone H2A.X(Ser139) Rabbit mAb [15L14K33]

Sample: Paraformaldehyde-fixed, paraffin embedded human

colorectal carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer

(pH 9.0) for 30 mins.

Primary antibody: R010101 at 1:200 dilution

Secondary antibody: Goat AntiRabbit lgG (H+L)

HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence-Anti-Phospho-Histone H2A.X (Ser139)

Rabbit mAb[15L14K33]

Sample: Hela cells

The cells were fixed with 4% paraformaldehyde (10 min)

permeabilized with 0.1%

Triton X-100 for 5 minutes and then blocked with 5% BSA in

0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R010101 at 1:100 dilution and α-tubulin

Mouse MonoclonalAntibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at

1:1,000 dilution (shown in green)

and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPl (shown in blue)