EZ RNA Methylation Kit (200 prep),货号-规格：R5002-200次

￥ 5996.00

￥ 8565.00

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EZ RNA Methylation Kit (200 prep),货号-规格：R5002-200次

HIGHLIGHTS

Specifically optimized for complete conversion of non-methylated cytosines in RNA.
Ideal for all RNA inputs.
Complete conversion of RNA in as little as 1 hour.

DESCRIPTION

The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent is provided ready-to-use: simply add the reagent to an RNA sample and incubate as indicated. The innovative in-column desulphonation technology eliminates messy precipitation steps, ensuring researchers obtain consistent results. The product has been designed to minimize template degradation, loss of RNA during treatment and clean-up, and to provide complete conversion of cytosine for accurate methylation analysis. Recovered RNA is ideal for RT-PCR, sequencing, library preparation and Next-Gen sequencing.

Conversion	> 99%
Elution Volume	≥ 10 µl
Equipment	Thermocycler with heated lid and microcentrifuge.
Input	0.5 - 1 µg of RNA.
Processing Time	50 Minutes
Purity	Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Recovery	> 80%

KIT COMPONENTS

Cat #	Name	Size
W1001-1	DNase/RNase-Free Water	1 ml
W1001-4	DNase/RNase-Free Water	4 ml
R5001-1-1	RNA Conversion Reagent	1.5 ml
R5001-3-10	RNA Desulphonation Buffer	10 ml
R5001-3-40	RNA Desulphonation Buffer	40 ml
C1004-50	Zymo-Spin IC Columns	50 Pack
C1001-50	Collection Tubes	50 Pack
R1013-2-25	RNA Binding Buffer	25 ml
R1003-3-12	RNA Wash Buffer	12 ml
R1003-3-24	RNA Wash Buffer	24 ml
R1013-2-100	RNA Binding Buffer	100 ml