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Direct-zol-96 RNA (4x96 Preps),货号-规格：R2056-4*96次

Direct-zol-96 RNA

HIGHLIGHTS

ü Easy Handling: Bypass chloroform, phase separation and precipitation steps.

ü NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).

ü Non-Biased: Complete RNA recovery without miRNA loss.

DESCRIPTION

The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).

TECHNICAL SPECIFICATIONS

PRODUCT FAQ

Q1: Is DNase I available for individual purchase?

All kit components are available for purchase separately.

Q2: How to store DNase-I following resuspension?

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

Q3: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q4: Is the kit compatible with samples stored in DNA/RNA Shield?

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

Q5: Is Direct-zol suitable for very small numbers of cells?

Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol.

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

Q10: Which phenol-based reagents are compatible with Direct-zol?

The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

PRODUCT   VIDEO

CITATIONS

Increased canonical NF-kappaB signaling specifically in macrophages is s...

BMC CancerPublished 7 Oct 2020

A genome assembly and the somatic genetic and epigenetic mutation rate i...

Genome BiolPublished 6 Oct 2020

Evolutionary dynamics of Tomato spotted wilt virus within and between al...

Sci RepPublished 25 Sep 2020

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REVIEWS

“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

- Adina B. (University of Guelph)

“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

- R.K. CSU

“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

- H.Z. (Harvard Medical School)

（More...）

KIT COMPONENTS

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