￥
7276.50
￥ 8085.00
BRIC Kit,货号-规格：RN1008-20 assays
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- 产品参数
- 说明书下载
试剂盒构成：
试剂 | 规格 |
| [RN1008](保存温度 2-8°C) | |
| 1. RNA-IP buffer | 18mL x 2 bottles |
| 2. Wash buffer | 41 mL x 3 bottles |
| 3. mi-Solution I | 240 ul x 1 vial: enzyme solution |
| 4. mi-Solution II | 5.8 mL x 1 vial: diluent for Solution 1 |
| 5. mi-SolutionIII | 3.6 mL x 1 vial: protein dissolvent |
| Solution III can dissolve proteins and dissociate immunocomp lex. | |
| 6. mi-Solution IV | 90 pL x 1 vial: co-precipitator |
| Solution IV can increase RNA precipitation efficiently. | |
| 7. Protein G-Magnetic beads | 1.5 mL x 4 vials |
| 1% beads slurry (mouse IgG binding capacity: 7 ug/mg beads) | |
| [RN1007]（保存温度-20℃） | |
| 8. BrU solution (100 mM) | 1.1 mL x 2 vials |
| 9. Anti-BrdU mAb | 450 ul x 1vial |
| 10. Spike-in control | 80 ul x 1 vial |
产品简介：
何为BRIC（5-Bromouridine Immunoprecipitation Chase）?
RNA 降解是调节细胞内 RNA 量和去除异常 RNA的重要机制。近年来，研究者发现人类基因的5-10%是通过 RNA 降解进行表达调控的，因此在基因表达调节中，RNA 降解与转录和翻译一样，受到人们的关注。BRIC是一种将5-溴尿苷（BrU）对细胞进行脉冲，并利用抗体回收所获得的 BrU标记 RNA 的方法。与原来使用转录抑制剂等方法不同，BRIC对细胞的不良影响小，较少引起 RNA 降解，因此作为一种可以准确获取数据的方法，受到人们广泛关注。BRIC是由东京大学同位素综合中心的秋光信佳教授等人发明的专利技术。（专利第5791140号）
将 BRIC与 RT-qPCR、微阵列、测序组合使用，可以分析细胞内目的 RNA量的变化以及探索新的功能性分子。
参考文献：
〈参考文献〉
1.Maekawa S etal. Analysis of RNA decay factor mediated RNA stability contributions on RNA abundance. BMC Genomics 16,154 (2015) (PMID: 25879614)
这是一篇通过比较了RNA-seq, ChlP-seq, BRIC-seq的数据，证明RNA的分解调控在基因表达调控中起到重要作用的文献。
2.Tani H etal. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947-56 (2012) (PMID: 22369889)
n这是一篇把BRIC和旧方法进行了比较，通过B方法鉴定了分解速度很快的RNA的文献。
3.Tani H, Akimitsu N. Genome-wide technology for determining RNA stability in mammalian cells: historical perspective and recent advantages based on modified nucleotide labeling. RNA Biol. 9,1233-8 (2012) (PMID: 23034600)
4.Imamachi N et al. BRIC-seq: a genome-wide approach for determining RNA stability in mammalian cells. Methods 67, 55-63 (2014) (PMID: 23872059)
5.Tani H etal The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells. PLoS One 8, e55684 (2013) (PMID: 23383264)
6.Tani H etaf. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012) (PMID: 230G4U4)
1.Maekawa S etal. Analysis of RNA decay factor mediated RNA stability contributions on RNA abundance. BMC Genomics 16,154 (2015) (PMID: 25879614)
这是一篇通过比较了RNA-seq, ChlP-seq, BRIC-seq的数据，证明RNA的分解调控在基因表达调控中起到重要作用的文献。
2.Tani H etal. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947-56 (2012) (PMID: 22369889)
n这是一篇把BRIC和旧方法进行了比较，通过B方法鉴定了分解速度很快的RNA的文献。
3.Tani H, Akimitsu N. Genome-wide technology for determining RNA stability in mammalian cells: historical perspective and recent advantages based on modified nucleotide labeling. RNA Biol. 9,1233-8 (2012) (PMID: 23034600)
4.Imamachi N et al. BRIC-seq: a genome-wide approach for determining RNA stability in mammalian cells. Methods 67, 55-63 (2014) (PMID: 23872059)
5.Tani H etal The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells. PLoS One 8, e55684 (2013) (PMID: 23383264)
6.Tani H etaf. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012) (PMID: 230G4U4)
实验数据：
品牌
MBL
规格
20 assays
价格
￥7276.50
市场价
￥8085.00
库存量
100