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Human TNF-α (肿瘤坏死因子α) ELISA kit,货号:CHE0019
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Human TNF-α (肿瘤坏死因子α) ELISA kit,货号-规格:CHE0019-96 wells


Overview

Product nameHuman TNF-α ELISA KIT
Catalog noCHE0019
Detection methodColorimetric
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity7 pg/mL
Assay range15.625 - 1000 pg/mL
Assay length3.5 hours
Assay procedureMultiple steps standard assay
SpecificityReacts with: Human
Product informationThe Human TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of TNF-α in human plasma, serum, and cell culture samples.This assay employs a quantitative sandwich enzyme immunoassay technique that measures human TNF-α in approximately 3.5 hours. A polyclonal antibody specific for human TNF-α has been pre-coated onto a 96-well microplate with removable strips. TNF-α in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TNF-α, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Background
Tested applicationSuitable for: Sandwich ELISA
FormatPre-coated microplate (12 x 8 well strips)
Properties
StorageStore at 2 – 8℃. Please refer to protocols.
Components1 x 96 tests
Antibody-coated microplate1 unit
Standard (Lyophilized)1 x 2 vials
Antibody conjugate concentrate1 x 2 vials
Streptavidin-HRP solution1 x 2 vials
Standard/sample diluent1 bottle
Antibody diluent buffer1 bottle
Streptavidin-HRP diluent1 bottle
Wash Buffer Concentrate 20X1 bottle
Substrate solution1 vial
Stop solution1 vial
Adhesive Films4 pieces


image of Human TNF-α ELISA KIT

Representative standard curve for TNF-α ELISA. TNF-α was diluted in serial two-fold steps in Sample Diluent.

文献引用:

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期刊影响因子:2.339
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期刊影响因子:2.066
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期刊:Nucleic Acids Research
期刊影响因子:11.147
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文章题目:Reducing postsurgical exudate in breast cancer patients by using San Huang decoction to ameliorate inflammatory status: a prospective clinical trial.
期刊:Current Oncology
期刊影响因子:1.967
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作者单位:南京中医药大学附属医院
发表时间:2018
原文链接:http://current-oncology.com/index.php/oncology/article/view/4108
文章题目:Circulating alarin concentrations are high in patients with type 2 diabetes and increased by glucagon-like peptide-1 receptor agonist treatment.
期刊:Medicine
期刊影响因子:1.87
作者:Wenjing Hu, Xiaoyun Fan, Baoyong Zhou etc.
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发表时间:2019
原文链接:https://journals.lww.com/md-journal/Fulltext/2019/07120/Circulating_alarin_concentrations_are_high_in.77.aspx
文章题目:Circulating Zbed3 Levels in Subjects With and Without Metabolic Syndrome.
期刊:Metabolic Syndrome and Related Disorders
期刊影响因子:1.744
作者:Wenjing Hu, Bo Tian, Xiaoqiang Li, etc.
作者单位:重庆市职业病防治医院
发表时间:2017
原文链接:https://www.liebertpub.com/doi/abs/10.1089/met.2016.0122
文章题目:A study on the immunomodulation of polysaccharopeptide through the TLR4-TIRAP/MAL-MyD88 signaling pathway in PBMCs from breast cancer patients
期刊:Immunopharmacology and Immunotoxicology
期刊影响因子:1.109
作者:Jing Wang, Bing Dong, Yi Tan, etc.
作者单位:重庆医科大学附属第二医院甲状腺胰外科临床实验室
发表时间:2013
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文章题目:Comparison between Dexmedetomidine and Propofol on the Inflammatory Responses in Elderly Patients Undergoing Radical Gastrectomy.
期刊:Investigacion Clinica
期刊影响因子:0.25
作者:Dandan Feng, Yue Liu, Xuan Luo, etc.
作者单位:南京医科大学南京鼓楼医院临床学院麻醉科
发表时间:2019
原文链接:888-Article%20Text-926-1-10-20190929.pdf














品牌
四正柏
价格
4550.00
市场价
2200.00
货期
3天
库存量
999
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