Human TNF-α (肿瘤坏死因子α) ELISA kit,货号-规格:CHE0019-96 wells
Overview
| Product name | Human TNF-α ELISA KIT |
| Catalog no | CHE0019 |
| Detection method | Colorimetric |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 7 pg/mL |
| Assay range | 15.625 - 1000 pg/mL |
| Assay length | 3.5 hours |
| Assay procedure | Multiple steps standard assay |
| Specificity | Reacts with: Human |
| Product information | The Human TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of TNF-α in human plasma, serum, and cell culture samples.This assay employs a quantitative sandwich enzyme immunoassay technique that measures human TNF-α in approximately 3.5 hours. A polyclonal antibody specific for human TNF-α has been pre-coated onto a 96-well microplate with removable strips. TNF-α in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human TNF-α, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. |
| Background | |
| Tested application | Suitable for: Sandwich ELISA |
| Format | Pre-coated microplate (12 x 8 well strips) |
| Properties | |
| Storage | Store at 2 – 8℃. Please refer to protocols. |
| Components | 1 x 96 tests |
| Antibody-coated microplate | 1 unit |
| Standard (Lyophilized) | 1 x 2 vials |
| Antibody conjugate concentrate | 1 x 2 vials |
| Streptavidin-HRP solution | 1 x 2 vials |
| Standard/sample diluent | 1 bottle |
| Antibody diluent buffer | 1 bottle |
| Streptavidin-HRP diluent | 1 bottle |
| Wash Buffer Concentrate 20X | 1 bottle |
| Substrate solution | 1 vial |
| Stop solution | 1 vial |
| Adhesive Films | 4 pieces |
image of Human TNF-α ELISA KIT

Representative standard curve for TNF-α ELISA. TNF-α was diluted in serial two-fold steps in Sample Diluent.
文献引用:
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| 发表时间: | 2016 |
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| 发表时间: | 2018 |
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| 发表时间: | 2019 |
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| 期刊: | ACS Nano |
| 期刊影响因子: | 13.903 |
| 作者: | Hongyu Wang, Xueting Pan, Xiaotong Wang, etc. |
| 作者单位: | 北京化工大学北京软物质科学与工程高精尖创新中心 |
| 发表时间: | 2020 |
| 原文链接: | https://www.ncbi.xilesou.top/pubmed/31909977 |
| 文章题目: | TERC promotes cellular inflammatory response independent of telomerase. |
| 期刊: | Nucleic Acids Research |
| 期刊影响因子: | 11.147 |
| 作者: | Haiying Liu, Yiding Yang, Yuanlong Ge, etc. |
| 作者单位: | 中山大学生命科学学院 |
| 发表时间: | 2019 |
| 原文链接: | https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz584/5531183 |
| 文章题目: | Reducing postsurgical exudate in breast cancer patients by using San Huang decoction to ameliorate inflammatory status: a prospective clinical trial. |
| 期刊: | Current Oncology |
| 期刊影响因子: | 1.967 |
| 作者: | Z. Y. Zhu, J. X. Xue, L. X. Yu, etc. |
| 作者单位: | 南京中医药大学附属医院 |
| 发表时间: | 2018 |
| 原文链接: | http://current-oncology.com/index.php/oncology/article/view/4108 |
| 文章题目: | Circulating alarin concentrations are high in patients with type 2 diabetes and increased by glucagon-like peptide-1 receptor agonist treatment. |
| 期刊: | Medicine |
| 期刊影响因子: | 1.87 |
| 作者: | Wenjing Hu, Xiaoyun Fan, Baoyong Zhou etc. |
| 作者单位: | 重庆市职业病防治医院 |
| 发表时间: | 2019 |
| 原文链接: | https://journals.lww.com/md-journal/Fulltext/2019/07120/Circulating_alarin_concentrations_are_high_in.77.aspx |
| 文章题目: | Circulating Zbed3 Levels in Subjects With and Without Metabolic Syndrome. |
| 期刊: | Metabolic Syndrome and Related Disorders |
| 期刊影响因子: | 1.744 |
| 作者: | Wenjing Hu, Bo Tian, Xiaoqiang Li, etc. |
| 作者单位: | 重庆市职业病防治医院 |
| 发表时间: | 2017 |
| 原文链接: | https://www.liebertpub.com/doi/abs/10.1089/met.2016.0122 |
| 文章题目: | A study on the immunomodulation of polysaccharopeptide through the TLR4-TIRAP/MAL-MyD88 signaling pathway in PBMCs from breast cancer patients |
| 期刊: | Immunopharmacology and Immunotoxicology |
| 期刊影响因子: | 1.109 |
| 作者: | Jing Wang, Bing Dong, Yi Tan, etc. |
| 作者单位: | 重庆医科大学附属第二医院甲状腺胰外科临床实验室 |
| 发表时间: | 2013 |
| 原文链接: | https://www.tandfonline.com/doi/abs/10.3109/08923973.2013.805764 |
| 文章题目: | Comparison between Dexmedetomidine and Propofol on the Inflammatory Responses in Elderly Patients Undergoing Radical Gastrectomy. |
| 期刊: | Investigacion Clinica |
| 期刊影响因子: | 0.25 |
| 作者: | Dandan Feng, Yue Liu, Xuan Luo, etc. |
| 作者单位: | 南京医科大学南京鼓楼医院临床学院麻醉科 |
| 发表时间: | 2019 |
| 原文链接: | 888-Article%20Text-926-1-10-20190929.pdf |