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Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease activity of proofreading DNA polymerases, such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.
Highlights
• 3’-terminal PTO modifications protect from 3’→5’ exonuclease degradation and ensure efficient priming of DNA synthesis
Applications
• Strand displacement amplification of genomic DNA (see Reference 2), plasmids and phage DNA
• DNA labeling by random priming (see References 4-6)
Note
• Use of this enzyme in certain applications may be covered by patents and may require a license.