规格

适用于（应用）:	Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
纯化目标:	Plasmid DNA
样品类型:	Bacteria
反应次数:	25 Preps
高通量能力:	Not High-throughput Compatible (Manual)
制备规模:	100-200 µg (Medium-Scale) Plasmid DNA
产品线:	GeneJET

描述

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified—> 400 ng/ µL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines

品牌

Thermo Scientific

规格

100 rxns

价格

￥2662.00

市场价

￥5915.00

库存量

100

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