￥
655.90
￥ 937.00
Endonuclease V, T.maritima (5 U/µL),货号-规格：EN0141-250U (5U/ul)
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Endonuclease V, T.maritima (5 U/µL),货号-规格：EN0141-250U (5U/ul)
描述
Thermo Scientific Endonuclease V, T. maritima (Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3' to a lesion.
Highlights
• Optimal activity at temperatures of 65 to 70°C
Applications
• High-throughput methods for mutation research
• Studies in mutagenesis and DNA repair
• Mismatch cleavage
• Genotyping
Note
Use of this enzyme in certain applications may be covered by patents and may require a license.
When the enzyme is in excess, the primary nicked products experience a second nicking event on the complementary strand, leading to a double-stranded break. At low concentrations, however, Endonuclease V first nicks a DNA strand at the lesions located closer to the 5'-end of DNA molecule. Single-stranded DNA is cleaved with much lower efficiency. Mg2+ or Mn2+ ions are required for enzyme activity.
品牌
Thermo Scientific
规格
250U (5U/ul)
价格
￥655.90
市场价
￥937.00
库存量
100