Bioss：IHC应用热门推荐抗体
什么是免疫组化？
免疫组织化学 (Immunocytochemistry, IHC) 是一门利用抗原与抗体特异性结合的原理，通过酶标抗体与底物液反应显色的技术。能对组织细胞内抗原进行定位、定性及定量的研究。由于免疫组化具有特异性强、灵敏度高、定位准确等特点，且能将形态研究与功能研究有机地结合在一起，因此在临床病理诊断中，免疫组织化学 (IHC) 是一种很重要的技术和手段。
免疫组化的作用
随着技术的发展，免疫组化被广泛应用于医学研究、生物学检测和病理诊断等许多领域，包括：
● 了解生物标志物和差异表达蛋白在生物组织不同部位的分布和定位；
● 恶性肿瘤的鉴别与诊断，对某些肿瘤进行进一步的分期和分级，了解分化程度；
● 转移瘤的原发部位或者起源的判定；
● 发现微小病灶，寻找原发瘤，指导临床治疗方案及手术范围选择；
● 指导靶向药物开发及适用疾病选择，临床治疗中靶向药物使用；
● 辨认细胞产物，对内分泌肿瘤作功能分类，检测分泌异位激素的肿瘤。
IHC 实验流程及配套试剂
Bioss 具有丰富的抗体研发经验，产品线包括已建立的常见标志物和潜在价值的新生标志物，涵盖肿瘤、神经系统疾病、神经退行性疾病和传染病等多种疾病领域。
科研免疫组化抗体的研发，生产和质检都遵循严格的标准，抗体的验证均经过切片检测，明确组织定位和细胞定位，抗体特异性强，灵敏度高，背景低。
| 疾病类型 | 相关靶点 |
| Aging | ● Tau ● TARDBP ● SNCA ● SOD1 |
| 肺癌 | ● GSTP1 ● MCM7 ● Ki-67 |
| 结直肠癌 | ● CALB2 ● GPA33 ● CK20 ● MUC2 ● Villin |
| 卵巢癌 | ● P53 ● CK7 ● Vimentin |
| 前列腺癌 | ● PSA ● ACP3 ● FOLH1 ● KRT5 |
| 乳腺癌 | ● ESR1 ● Caveolin-1 ● SMMHC ● KRT14 ● Actin |
| ● PGR ● GATA3 ● CADH1 Alpha Smooth Muscle | |
| 肾癌 | ● CD10 ● Pan CK ● CD20 |
| 胰腺癌 | ● S100P ● MSLN ● TYMS ● ERCC1 ● MUC1 |
| ● CEACAM1 ● MUC-4 |
抗体经过不同种属组织样本全面验证，包括阳性对照和阴性对照。
抗体通过反复的筛选和测试，确保灵敏度更高，背景更低，染色更清晰，定位更精准。
非特异性染色低，染色结果更为准确。
抗体稀释比至少 1:100 以上，部分甚至可达 1:1000。
生产的可重复性、一致性高，批次间差异小，抗体的重复性更好。
具有保存稳定性，运输稳定性，反复冻融稳定性。
IHC 应用热门推荐抗体验证数据
bsm-33042M
Histone H3 Mouse mAb
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
bsm-33170M
Vimentin Mouse mAb
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site
of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
IHC应用热门推荐抗体产品列表（部分）
Product Name | Catalog # | Reactivity (IHC) | Application | PUBMED |
5 Lipoxygenase Recombinant Rabbit mAb | bsm-61061R | Human,Rat | WB,IHC-P,IHC-F,IF | |
AACT Recombinant Rabbit mAb | bsm-60780R | Human | WB,IHC-P,IHC-F,IF | |
ACE2 Rabbit pAb | bs-46001R | Human | WB,IHC-P,IHC-F,IF | |
ACE2 Recombinant Rabbit mAb | bsm-52614R | Rat,Mouse,Human | WB,IHC-P,IHC-F,ICC/IF,IF | * |
ACTH Mouse mAb | bsm-34147M | Human | IHC-P,IF,IHC-F | |
Actin, alpha skeletal muscle Mouse mAb | bsm-33297M | Human,Rat | WB,IHC-P,IHC-F,IF | |
AFP Recombinant Rabbit mAb | bsm-60789R | Human | WB,IHC-P,IF,IHC-F | |
AFP(A2) Mouse mAb | bsm-1621M | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF,ELISA | * |
AIF1 (9A3) Recombinant Rabbit mAb | bsm-54132R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | * |
AKT1 Recombinant Rabbit mAb | bsm-52010R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | * |
ALDH1A1 Recombinant Rabbit mAb | bsm-60321R | Human,Mouse | WB,IHC-P,IHC-F,IF | |
bsm-60542R | Human,Mouse,Rat | WB,IHC-P,IHC-F,ICC/IF,IF | ||
alpha smooth muscle Actin Mouse mAb | bsm-33187M | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF,Flow-Cyt | * |
alpha smooth muscle Actin Rabbit pAb | bs-10196R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF,Flow-Cyt | * |
alpha smooth muscle Actin Recombinant Rabbit mAb | bsm-52392R | Human,Mouse,Rat | WB,IHC-P,IHC-F,ICC/IF,IF | * |
Alpha Synuclein Recombinant Rabbit mAb | bsm-54277R | Human | WB,IHC-P,IHC-F,ICC/IF,IF,Flow-Cyt | * |
Androgen receptor Recombinant Rabbit mAb | bsm-61204R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | |
AQP1 Recombinant Rabbit mAb | bsm-52909R | Human,Mouse,Rat | WB,IF,IHC-P,IHC-F | |
AQP4 Recombinant Rabbit mAb | bsm-61035R | Human,Rat,Mouse | IHC-P,IF,IHC-F | |
AR/Androgen receptor Mouse mAb | bsm-34141M | Human | IHC-P,IHC-F,IF | * |
Arginase 1 Mouse mAb | bsm-34185M | Human | IHC-P,IHC-F,IF | |
Arginase 1 Recombinant Rabbit mAb | bsm-60019R | Human | WB,IHC-P,IHC-F,IF | |
ATF4 Rabbit pAb | bs-1531R | Human,Mouse,Rat | WB,IHC-P,IHC-F,ICC/IF,IF,Flow-Cyt | * |
ATP1A1 Rabbit pAb | bs-42166R | Mouse,Human,Rat | IHC-P,IF,IHC-F | * |
ATP1B1 Recombinant Rabbit mAb | bsm-61065R | Human,Mouse,Rat | IHC-P,IF,IHC-F | |
Bax Recombinant Rabbit mAb | bsm-52316R | Rat,Mouse,Human | WB,IHC-P,IHC-F,IF | * |
Bcl-2 Mouse mAb | bsm-33411M | Human,Rat | WB,IHC-P,IHC-F,ICC/IF,IF | * |
Bcl-2 Recombinant Rabbit mAb | bsm-61074R | Human,Mouse,Rat | IHC-P,IHC-F,IF,WB | * |
Bcl-6 Rabbit pAb | bs-2734R | Human,Mouse | WB,IHC-P,IHC-F,IF,ELISA | * |
Beta catenin Mouse mAb | bsm-36202M | Human,Rat | IF,IHC-P,IHC-F | * |
Beta tubulin Mouse mAb | bsm-33120M | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | |
Beta tubulin Mouse mAb, Loading Control | bsm-33034M | Human,Mouse,Rat | WB,IHC-P,IHC-F,ICC/IF,IF | * |
Beta tubulin Rabbit pAb, Loading Control | bs-4511R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF,Flow-Cyt | * |
Beta tubulin Recombinant Rabbit mAb | bsm-52290R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | * |
Beta-Amyloid (1-42) Mouse mAb | bsm-41470M | Human | WB,IHC-P,IHC-F,IF | * |
BOB1 Mouse mAb | bsm-34182M | Human | IHC-P,IHC-F,IF | |
BRAF Rabbit pAb | bs-23436R | Human,Mouse,Rat | IHC-P,IHC-F,IF | * |
BRD7 Rabbit pAb | bs-7779R | Human,Mouse,Rat | IHC-P,IHC-F,IF | |
CA9 Mouse mAb | bsm-33082M | Human | IHC-P,IHC-F,IF | * |
Cadherin Recombinant Rabbit mAb | bsm-63043R | Human,Mouse,Rat | IHC-P,IHC-F,IF,WB | |
Calbindin Rabbit pAb | bs-10177R | Human,Mouse,Rat | WB,IHC-P,IHC-F,ICC/IF,IF | |
Calbindin Recombinant Rabbit mAb | bsm-60713R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | |
Caldesmon Recombinant Mouse mAb | bsm-60332M | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | |
Caldesmon/CDM Recombinant Rabbit mAb | bsm-60918R | Human | IHC-P,IHC-F,IF | |
Calreticulin | bs-5913R | Human | WB,IHC-P,IHC-F,IF,ELISA | * |
Calreticulin Mouse mAb | bsm-51748M | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | * |
Calreticulin Recombinant Rabbit mAb | bsm-52436R | Human,Mouse,Rat | WB,IHC-P,IHC-F,ICC/IF,IF,Flow-Cyt | * |
Calretinin Recombinant Rabbit mAb | bsm-54076R | Human,Mouse,Rat | WB,IHC-P,IHC-F,IF | |
Cardiac Troponin I/TNIC Mouse mAb | bsm-41212M | Human,Rat,Mouse | IHC-P,IHC-F,IF | |
Caspase-3 Recombinant Rabbit mAb | bsm-61071R | Human,Mouse,Rat | IHC-F,IHC-P,IF,WB | |
bsm-52289R | Human | WB,IHC-P,IHC-F,IF | * |
查看更多IHC应用热门推荐抗体产品列表：https://kdocs.cn/l/cvvF7Gx2LXHS
IHC 配套试剂表
分类 | 编号 | 名称 | 规格 | 价格 |
IHC试剂盒 | IHC001 | 免疫组化试剂盒（抗兔） | 60T/100T | 680/960 |
IHC003 | 免疫组化试剂盒（抗小鼠） | 60T/100T | 680/960 | |
取材 | C2019 | 蔗糖-PBS溶液（5%） | 500ml | 160 |
C2020 | 蔗糖-PBS溶液（10%） | 500ml | 160 | |
C2021 | 蔗糖-PBS溶液（20%） | 500ml | 160 | |
C2022 | 蔗糖-PBS溶液（30%） | 500ml | 180 | |
固定液 | C01-06001 | 1%组织细胞固定液 | 100ml/500ml | 60/180 |
C01-06002 | 4%组织细胞固定液 | 100ml/500ml | 60/200 | |
C01-06003 | 8％组织细胞固定液 | 100ml/500ml | 100/300 | |
C2058 | PFA-蔗糖溶液（5%） | 500ml | 300 | |
C2059 | PFA-蔗糖溶液（10%） | 500ml | 300 | |
C2060 | PFA-蔗糖溶液（20%） | 500ml | 300 | |
C2061 | PFA-蔗糖溶液（30%） | 500ml | 300 | |
C2023 | AAF固定液 | 100ml/500ml | 80/120 | |
C2026 | Carnoy固定液 | 100ml/500ml | 100/360 | |
C2027 | Carnoy固定液Ⅱ | 100ml/500ml | 120/360 | |
C2030 | Gendre固定液 | 100ml/500ml | 300/860 | |
C2035 | Orth固定液 | 500ml | 360 | |
C2040 | Regaud固定液 | 500ml | 240 | |
C2041 | Rossman固定液 | 500ml | 360 | |
C2034 | 中性组织细胞固定液（10%,NBF） | 500ml/500ml×10 | 200/1680 | |
C2055 | 组织细胞固定液（4%, RNase free） | 10×10ml/500ml | 200/240 | |
C2056 | 组织细胞固定液（4% PFA,电镜专用） | 10×10ml/10×30ml | 200/300 | |
C2064 | 脂肪组织固定液 | 250ml | 520 | |
脱钙液 | C2003 | Plank-Rychlo脱钙液 | 500ml | 280 |
C2004 | Perenyi's脱钙液 | 500ml | 500 | |
C2005 | Von Ebener's脱钙液 | 500ml | 400 | |
C2006 | 甲酸脱钙液 | 500ml | 280 | |
C2008 | 柠檬酸盐脱钙液 | 500ml | 220 | |
C2010 | JYBL-Ⅱ脱钙液 | 500ml | 180 | |
C2007 | AFIP甲酸-柠檬酸液 | 2×500ml | 500 | |
C2013 | 电解脱钙液（甲酸法） | 500ml | 220 | |
C2016 | 硫酸钠水溶液（5%） | 500ml | 180 | |
C2017 | 脱钙终点检测试剂盒（化学法） | 100T | 280 | |
切片 | C2078 | OCT冰冻切片包埋剂 | 118ml/118ml×6/118ml×12 | 198/1080/1900 |
C02-01001 | 多聚赖氨酸溶液（防脱片粘片剂） | 5ml/10ml/100ml | 160/280/2200 | |
染色 | C02-04002 | DAPI染色液（即用型） | 10ml/50ml | 120/320 |
S0001 | DAPI染色液（1mg/ml） | 1ml/10×1ml | 200/1200 | |
C02-04004 | 苏木素-伊红染色试剂盒 | 10ml/100ml | 130/320 | |
抗原修复液 | C2081 | 胰蛋白酶消化液（0.1% 用于抗原修复） | 10ml | 120 |
C02-02001 | 1×柠檬酸钠抗原修复液（干粉） | 1L/1L×10 | 10/90 | |
C02-02002 | 柠檬酸钠抗原修复液（100×） | 50ml/100ml/500ml | 90/150/360 | |
C02-02003 | 1×EDTA抗原修复液（pH8.0） | 100ml | 60 | |
C02-02004 | 50×EDTA抗原修复液（pH8.0） | 100ml | 90 | |
C5077 | 细胞爬片抗原修复液（即用型） | 100ml/500ml | 100/300 | |
C-0012 | 胰蛋白酶抗原修复液 | 3ml/10ml | 180/348 | |
C2082 | 冰冻切片抗原修复液 | 100ml/500ml | 120/300 | |
洗涤剂 | C01-01001 | 1×PBS缓冲液（细胞培养级别） | 500ml/500ml×10 | 70/600 |
C01-01002 | 10×PBS缓冲液 (pH7.2- 7.4) | 50ml/500ml/500ml×10 | 60/80/700 | |
C01-01003 | 1×PBS缓冲液（干粉） | 1L/1L×10 | 10/80 | |
C0103 | 1×PBS缓冲液（干粉，2L） | 2L/2L×10/2L×100 | 10/80/760 | |
C01-01004 | 1×PBST缓冲液 | 500ml/500ml×10 | 60/500 | |
穿膜剂 | C03-02002 | 穿膜剂（储存液） | 50ml | 160 |
C03-03002 | 0.3%细胞穿膜剂（即用型） | 100ml | 40 | |
C03-03003 | 0.1%细胞穿膜剂（即用型） | 100ml/500ml | 40/120 | |
封闭用血清 | C-0005 | 封闭用正常山羊血清（原液） | 5ml/10ml/100ml | 40/70/400 |
C-0006 | 封闭用正常兔血清（原液） | 5ml/10ml | 40/70 | |
C-0008 | 封闭用驴血清（原液） | 10ml/100ml | 100/680 | |
C-0082 | 封闭用正常小鼠血清（原液） | 5ml | 280 | |
C01-03001 | 10%封闭山羊血清 | 30ml/100ml | 50/100 | |
抗体稀释液 | C01-04001 | 通用型抗体稀释液（PBS） | 30ml/100ml/500ml | 80/200/500 |
C01-04002 | HRP标记抗体稀释液 | 30ml/60ml/100ml | 80/140/240 | |
C01-04003 | AP标记抗体稀释液 | 30ml/50ml/100ml | 80/150/280 | |
C01-04004 | 荧光抗体稀释液 | 30ml/100ml | 50/120 | |
C04-01005 | 酶标抗体稀释液 | 30ml/50ml/100ml | 80/140/240 | |
检测试剂盒 | PV-1022 | 超敏二步法免疫组化检测试剂盒（兔鼠通用，即用型） | 3ml/6ml/50ml | 480/880/6400 |
PV-1023 | 超敏二步法免疫组化检测试剂盒（兔，即用型） | 3ml/6ml/50ml | 480/880/6400 | |
PV-1024 | 超敏二步法免疫组化检测试剂盒（小鼠，即用型） | 3ml/6ml/50ml | 480/880/6400 | |
显色试剂盒 | S0339 | AEC显色试剂盒（20×） | 3×1ml/3×10ml | 160/980 |
C-0010 | 二氨基联苯胺/DAB染色试剂盒（20×） | 0.5ml×2/1ml×2 | 70/120 | |
C-0014 | BCIP/NBT碱性磷酸酶显色试剂盒（即用型） | 25ml/125ml | 180/320 | |
封片剂 | C02-04003 | 抗荧光淬灭封片液/抗荧光衰减封片剂 | 5ml | 280 |
C02-05001 | 中性树胶封片剂 | 10ml/100ml | 50/180 | |
阴性对照 | bs-0294PC | 正常山羊对照IgG | 100ug/500ug | 98/280 |
bs-0295PC | 正常兔对照IgG | 100ug/500ug | 98/280 | |
bs-0296PC | 正常小鼠对照IgG | 100ug/500ug | 148/380 | |
bs-0310PC | 正常鸡对照IgG | 100ug/500ug | 148/480 | |
bs-0326PC | 正常牛对照IgG | 100ug/500ug | 98/280 | |
bs-0358PC | 正常豚鼠对照IgG | 100ug/500ug | 148/480 |