10X CpG Reaction Buffer (1 ml),货号-规格:E2010-2-1kit
For complete, in vitro methylation of DNA for methylation analysis.
Methylation of chromatin DNA for DNA accessibility studies.
Inhibition of endonucleases with overlapping CpG sequence recognition and [3H]-labeling of DNA.
Additional Information | Enzyme Commission Number (EC 2.1.1.37) |
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Assay Condition | CpG Methylase in 1X CpG Reaction Buffer w/ 600 µM SAM. Incubate reaction mixtures at 30C (Performing the Methylase reaction at 30°C rather than 37°C promotes optimal reaction kinetics). |
Heat Inactivation | 65°C for 20 minutes |
Source | Recombinant methylase is isolated from E. coli expressing the methyltransferase gene from Spiroplasma sp. strain MQ1. |
Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles of reagents. Prolonged storage is at ≤ -70°C. |
Unit Definition | One unit (U) is the amount of enzyme required to protect 1µg of λ DNA from cleavage by BstUI restriction endonuclease in a total reaction volume of 20 µl for 1 hour at 37°C. |
KIT COMPONENTS
Cat # | Name | Size |
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E2010-2 | 10X CpG Reaction Buffer | 1 ml |
E2010-3 | 20X SAM (S-adenosylmethionine) | 200µl |